Related Experiment Video
Updated: Oct 21, 2026

Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis
Published on: February 8, 2010
Mutagenic and genotoxic effects of mate (Ilex paraguariensis) in prokaryotic organisms
1Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
Abstract:
1. The mutagenic and genotoxic effects of mate (Ilex paraguariensis) aqueous solutions were analyzed in bacterial cells. 2. Mate solutions showed mutagenic activity in the Ames test (TA97, TA98, TA100 and TA102 strains) at concentrations of 20 to 50 mg/plate (mutagenic factors of 3.5 to 5.6) and genotoxic activity in the inductest (WP2s (lambda) strain), with a maximal phage induction at concentrations of 10 to 20 mg/plate. Above these concentrations the mate solutions were cytotoxic. 3. Addition of 5 U/ml catalase, 20 microliters/ml S9 rat liver microsomal fraction, 100 mM thiourea or 10 mM dipyridyl completely inhibited the lysogenic induction produced by mate; however, the addition of 1,000 U/ml superoxide dismutase was almost ineffective. 4. Oxygen reactive species may be present in mate solutions playing an essential role in its genotoxicity.
Related Concept Videos
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...
In-vitro Mutagenesis (Gene Knockouts)
In vitro Mutagenesis
Mismatch Repair
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Mutations in Microorganisms
Spontaneous and Induced Mutations

