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Evidence against the existence of the purported Saccharomyces cerevisiae PKC2 gene
D E Levin1, W D Stevenson, M Watanabe
1Department of Biochemistry, Johns Hopkins University School of Public Health, Baltimore, Maryland 21205.
Background:
The existence of a Saccharomyces cerevisiae gene encoding a novel isoform of protein kinase C was reported recently in this journal.
Results:
We demonstrate here that, firstly, the purported PKC2 gene does not reside at the chromosomal location to which it was assigned; secondly, it does not exist as a contiguous sequence in the S. cerevisiae genome; thirdly, some of its reported sequences do exist within other yeast genes; and fourthly, some of its reported sequences, encoding regions of the predicted protein related to protein kinase C, do not exist in any context in the yeast genome.
Conclusions:
We conclude from these studies that the PKC2 gene is a composite construction of unrelated yeast and non-yeast sequences.