Release of slow axonally transported proteins from the rat vagus nerve in vitro

The cultured rat vagus nerve was used to investigate the release of [35S]methionine-labelled slow axonally transported proteins during regeneration. After metabolic labelling the released proteins were collected from an isolated compartment at the distal end of the nerve. Several proteins were released at a time point consistent with the arrival of slow axonally transported proteins at the collection compartment, including actin and a group of 150 kDa proteins.