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Related Experiment Videos

CD28 activation promotes Th2 subset differentiation by human CD4+ cells

C L King1, R J Stupi, N Craighead

  • 1Division of Geographic Medicine, Case Western Reserve University, Cleveland, OH 44106-4983.

European Journal of Immunology
|February 1, 1995
PubMed
Summary

CD28 activation enhances T helper cell production of IL-4 and IL-5, promoting a Th2 response. Repeated stimulation with CD28 drives Th2 differentiation in naive cells, independent of IL-4.

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Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • CD28 costimulation is crucial for T cell activation and IL-2 production.
  • The role of CD28 in T helper cell differentiation and other cytokine production is not fully understood.

Purpose of the Study:

  • To investigate the role of CD28 ligation in T helper (Th) cell cytokine production and differentiation.
  • To determine the impact of repetitive CD28 stimulation on Th cell responses.

Main Methods:

  • Highly purified human adult and neonatal CD4+ T cells were stimulated with anti-CD3, PMA/ionomycin, or PHA, with or without anti-CD28.
  • Cytokine production (IL-2, IL-4, IL-5, IFN-gamma) and mRNA levels were analyzed over repetitive stimulation cycles.
  • ELISPOT assays were used to quantify IL-4 secreting cells.

Related Experiment Videos

Main Results:

  • Initial CD28 stimulation augmented IL-2, IL-4, IL-5, and IFN-gamma production.
  • Repetitive CD28 stimulation progressively increased IL-4 and IL-5 production while decreasing IL-2 and IFN-gamma.
  • CD28 activation induced a Th2-like response in naive neonatal T cells, independent of IL-4.

Conclusions:

  • CD28 ligation plays a significant role in promoting Th2 cell differentiation in humans.
  • Repetitive CD28 stimulation can drive naive T cells towards a Th2 phenotype.
  • CD28-mediated Th2 induction is IL-4 independent.