[Effect of media conditioned with bone marrow fibroblasts on proliferation of leukemic blast cell lines]

The influence of condition media of bone marrow fibroblasts (BMF) from patients with acute and chronic leukemia (myeloid and lymphoid forms) on the proliferation of K-562, HL-60 and HL-60 leukemic cells treated with tetraphorbol ester inducing macrophage-like differentiation of these cells has been studied. It is shown that BMF inhibit proliferation of leukemic cells, the effect being both direct and mediated by autocrine secretion. BMF effect depends both on the disease specificity and the type of cell line tested. The inhibition effect of BMF on HL-60 also depends on the quantity of megakaryocytes in the primary bone marrow. The role of cell-cell interaction in the ability of bone marrow to influence leukemic blast cells is discussed.