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Related Experiment Videos

Programmed cell death in Dictyostelium

S Cornillon1, C Foa, J Davoust

  • 1Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France.

Journal of Cell Science
|October 1, 1994
PubMed
Summary
This summary is machine-generated.

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Early nucleolar disorganization in Dictyostelium cell death.

Cell death & disease·2017

Programmed cell death (PCD) in Dictyostelium discoideum exhibits unique timing, with irreversible changes occurring early and membrane permeabilization late. This suggests a conserved core mechanism for PCD across diverse species.

Area of Science:

  • Cell Biology
  • Evolutionary Biology
  • Developmental Biology

Background:

  • Programmed cell death (PCD) is crucial for development and tissue homeostasis.
  • Understanding the evolutionary origins and core mechanisms of PCD is a significant challenge.
  • Dictyostelium discoideum offers a model system to study PCD in a unicellular organism that can aggregate.

Purpose of the Study:

  • To quantitatively analyze the temporal progression of programmed cell death (PCD) in Dictyostelium discoideum.
  • To compare the characteristics of PCD in Dictyostelium with known apoptotic and non-apoptotic pathways.
  • To hypothesize a conserved core molecular mechanism for PCD across evolution.

Main Methods:

  • In vitro system for precisely triggering and studying PCD without morphogenesis.

Related Experiment Videos

  • Confocal microscopy to observe vacuolization.
  • Electron microscopy to identify cytoplasmic and chromatin condensation.
  • Propidium iodide staining to assess membrane permeabilization.
  • Gel electrophoresis (standard and pulsed field) to detect DNA fragmentation.

    • Main Results:

    • An irreversible step, leading to inability to regrow, was observed at 8 hours.
    • Massive vacuolization, cytoplasmic condensation, and chromatin condensation occurred by 12 hours.
    • Late membrane permeabilization (40-60 hours) and absence of early DNA fragmentation were noted.
    • PCD characteristics showed similarities to both apoptotic and non-apoptotic PCD.

    Conclusions:

    • Dictyostelium discoideum PCD shares traits with other PCD forms, suggesting a conserved core mechanism.
    • This core mechanism likely predates the emergence of multicellularity.
    • Phenotypic variations in PCD arise from differences in cellular enzymatic equipment and mechanical constraints.
    • A prediction is the evolutionary relatedness of molecules involved in PCD across distant species like Dictyostelium and vertebrates.