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False positive immunoreaction in products of conception
F G Barker1, P Ozua, S Van Noorden
1Department of Histopathology, Royal Postgraduate Medical School, London.
Journal of Clinical Pathology
|December 1, 1994
Summary
False positive cytomegalovirus immunostaining in conception products was caused by endogenous biotin. Using a non-avidin-biotin method successfully resolved this diagnostic issue.
Area of Science:
- Pathology
- Immunohistochemistry
- Reproductive Medicine
Background:
- Cytomegalovirus (CMV) detection in products of conception is crucial for diagnosing fetal infections.
- Immunohistochemical (IHC) methods are commonly used for CMV detection.
- Avidin-biotinylated peroxidase complex (AB Complex) is a widely used IHC detection system.
Purpose of the Study:
- To investigate the cause of false positive cytomegalovirus immunostaining in products of conception.
- To evaluate the efficacy of alternative IHC methods in resolving this issue.
Main Methods:
- Analysis of products of conception samples with suspected false positive CMV immunostaining.
- Utilized avidin-biotinylated peroxidase complex (AB Complex) method.
- Employed a non-avidin-biotin IHC method for comparison.
Main Results:
- False positive CMV immunostaining was identified in products of conception samples.
- Endogenous biotin in the samples was confirmed as the cause of false positivity with the AB Complex method.
- The non-avidin-biotin IHC method eliminated the false positive results.
Conclusions:
- Endogenous biotin interferes with avidin-biotin based IHC assays for CMV detection in products of conception.
- Non-avidin-biotin detection systems are recommended to avoid false positives in such cases.
- Accurate CMV diagnosis in products of conception requires careful selection of IHC methodology.