Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Changes in UsnRNA biosynthesis during rat liver regeneration

R Ray¹, C K Panda, B K Chakraborty

¹Department of Biochemistry, Chittaranjan National Cancer Institute, Calcutta, India.

Molecular and Cellular Biochemistry

|December 7, 1994

Summary

Following partial hepatectomy (PH), spliceosomal UsnRNAs (U1, U2, U4-U6) levels decrease, while U3 snRNA levels fluctuate. UsnRNA synthesis kinetics in regenerating hepatocytes mirror these content changes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Integrated machine learning-driven QSAR modelling and systems biology approach for the identification of potential SARS-CoV-2 3CLpro inhibitors.

SAR and QSAR in environmental research·2025

Same author

Fourth-Generation Percutaneous Transverse Osteotomies for Hallux Valgus.

The Journal of bone and joint surgery. American volume·2025

Same author

A systematic review of biomechanical studies utilising finite element analysis in hallux valgus deformity.

Foot and ankle surgery : official journal of the European Society of Foot and Ankle Surgeons·2025

Same author

Editorial Expression of Concern: Src kinase as a mediator of convergent molecular abnormalities leading to NMDAR hypoactivity in schizophrenia.

Molecular psychiatry·2025

Same author

Comparative biomechanical study of different screw fixation methods for minimally invasive hallux valgus surgery: A finite element analysis.

Foot and ankle surgery : official journal of the European Society of Foot and Ankle Surgeons·2024

Same author

Improvement in clinical outcomes following arthroscopic all-inside medial lateral ligament reconstruction for rotational ankle instability.

Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA·2024

Area of Science:

Molecular Biology
Cellular Biology
Biochemistry

Context:

Partial hepatectomy (PH) triggers liver regeneration, a process involving significant cellular and biochemical changes.
While morphological and major biochemical alterations are documented, the dynamics of small nuclear RNAs (UsnRNAs) during this phase remain less understood.

Purpose:

To investigate the alterations in the cellular content and synthesis rates of spliceosomal UsnRNAs (U1, U2, U4-U6) and U3 snRNA during liver regeneration after partial hepatectomy.

Summary:

Spliceosomal UsnRNA levels decreased by 30-50% within 48 hours post-PH, then gradually recovered. U3 snRNA showed distinct fluctuations, increasing significantly at 24 and 72 hours.
UsnRNA synthesis rates in regenerating hepatocytes initially increased by 20-30% at 24 hours, then decreased by 30-50% at 48 hours, followed by a subsequent rise from 72 hours onwards.

Related Experiment Videos

Impact:

This study reveals dynamic changes in UsnRNA levels and synthesis during liver regeneration, suggesting their involvement in the complex regulatory mechanisms of hepatic growth.
Understanding these UsnRNA dynamics provides insights into the molecular orchestration of liver repair and could have implications for regenerative medicine.