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Long-lived testosterone esters in the rat

W Borg1, C H Shackleton, S L Pahuja

  • 1Yale University School of Medicine, Department of Obstetrics and Gynecology, New Haven, CT.

Proceedings of the National Academy of Sciences of the United States of America
|February 28, 1995
PubMed
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Naturally occurring testosterone esters, similar to therapeutic synthetic versions, were found in rat fat and testes. These fatty acid esters act as a potential reservoir for androgens, impacting physiological functions.

Area of Science:

  • Endocrinology
  • Biochemistry

Background:

  • Steroid hormones, like testosterone, are increasingly recognized as being esterified with fatty acids.
  • These naturally occurring steroidal esters are analogous to synthetic esters used therapeutically.

Purpose of the Study:

  • To investigate the natural existence of testosterone esters in mammalian tissues.
  • To characterize the distribution and dynamics of testosterone esters in rats.

Main Methods:

  • Immunoassay to quantify nonpolar saponifiable metabolites (testosterone esters).
  • Gas chromatography-mass spectrometry (GC/MS) to confirm the presence of testosterone in hydrolyzed ester fractions.
  • Analysis of testosterone ester levels in male and female rats, including castrated males over time.

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Main Results:

  • Significant quantities of testosterone esters were found in the fat (9 ng/g) and testes (3 ng/g) of male rats.
  • Testosterone esters were absent in female rats and long-term castrated males.
  • GC/MS confirmed testosterone presence in ester fractions from intact males but not castrates.
  • Testosterone levels in fat disappeared within 6 hours of castration, while ester levels showed a delayed decline, persisting for over 10 days.

Conclusions:

  • This study demonstrates the existence of previously unrecognized natural testosterone esters.
  • Testosterone esters in adipose tissue may function as a reservoir of preformed androgens.
  • These findings suggest a potentially significant physiological impact of natural testosterone esters.