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A gene transfer method for the study of chimerism derived from donor bone marrow in organ transplant recipients
J P Smith1, J Kasten-Jolly, L Rebellato
1East Carolina University, School of Medicine, Department of Surgery, Greenville, North Carolina 27858.
Transplantation Proceedings
|February 1, 1995
Abstract:
Transduction of DBMC with the neo gene did not negatively affect the viability of DBMC or the graft-prolonging effect of DBMC infusion and provided a sensitive method to monitor DBMC-derived chimerism at a microchimeric level for at least 6 months by molecular and immunohistochemical techniques. Selection of neo-transduced DBMC with G418 prior to infusion will enable more uniform neo expression and will provide novel opportunities to further investigate chimerism in primates.