[The effect of hypothermia on certain biochemical indicators of isolated hepatocytes]

The media containing nonpenetrating components (0.25 M sucrose, BSA) and antioxidant (silibor) are able for a long time to preserve at 4 degrees C a great number of liver cells. Survival of the same hepatocytes after normothermic incubation indicated a good condition of the mitochondrial system both at the stage of isolation and after hypothermic storage. Thus, by inhibiting osmotic impairment, which developed under hypothermic storage, we produced a prolongation of the time of "ageing-death" of an isolated liver cell at 4 degrees C. The addition of a drug possessing antioxidant properties was also beneficial.