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Integration of synthetic globin genes into an E. coli plasmid
Nucleic Acids Research
|August 1, 1976
Abstract:
Rabbit globin mRNA has been purified and used as a template by reverse transcriptase. The resulting duplex molecule consisting of rabbit globin mRNA/cNDA has been linked in vitro to Eco RI cleaved plasmid Col E1 DNA. Transformation of E. coli C6OO by this recombinant molecule has been achieved. Transformed bacteria acquire the colicin EQ immunity of Col E1 and a closed circular DNA species of 4.40-4.45 x 10 (6) daltons in molecular weight, an increase of 2.0-2.5 x 10(5) daltons compared to that of the parent plasmid DNA. In addition , 3H cDNA synthesized from globin RNA hybridized perferentially to the recombinant plasmid DNA.