[Fluorescent in situ hybridization of enriched SFA-DNA on pachytene chromosomes in mouse]

In the present study, the primary constrictions of mouse pachytene chromosomes stained with Hoechst 33258, and those stained by indirect immunofluorescence method with CREST antiserum were compared with those hybridized by the probe DNA isolated from the enriched mouse spindle fiber attachments. It was found that the DNA probe hybridized not only with the centromeric regions on synaptonema complexes but also with the DNA of pericentromeric heterochromatin of all autosomes. Moreover, the DNA probe hybridized also with the centromeric regions of both X and Y chromosomes. It was concluded that the DNA isolated from the enriched mouse spindle fiber attachments contains a complete set of the centromeric DNAs of all autosomes, X and Y chromosomes.