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Enzyme immunoassay for the detection of levamisole in meat and milk
1Department of Biochemistry Pathology and Parasitology, Central Veterinary Laboratory, Addlestone, Surrey, UK.
The Analyst
|December 1, 1994
Abstract:
Ovine polyclonal antisera were raised to aminolevamisole to develop an enzyme immunoassay for levamisole. The assay has been validated in milk and in 100 g l-1 muscle homogenate. The limit of detection is 1 microgram l-1. In milk the inter-assay relative standard deviation (Sr) is 6% at 10 micrograms kg-1 and the intra-assay Sr value 7.2%. In tissue homogenate the inter-assay Sr value is 12.7% at 5 micrograms kg-1 and the intra-assay Sr value is 3.8%. Total incubation time is 1 h.