Related Experiment Videos
Epidermal growth factor excretion in burned rats
M Farriol1, S Schwartz, J Rossello
1Metabolic Research Unit Santiago Grisolia, Department of Biochemistry, Hospital General Vall d'Hebron, Barcelona, Spain.
Burns : Journal of the International Society for Burn Injuries
|December 1, 1994
Summary
Urinary epidermal growth factor (EGF) levels increased significantly in male rats after burn injury, suggesting EGF
Area of Science:
- Physiology
- Biochemistry
- Burn Medicine
Background:
- Epidermal growth factor (EGF) plays a role in wound healing.
- The physiological response to thermal injury is complex.
- Urinary markers can reflect systemic physiological changes.
Purpose of the Study:
- To investigate the role of EGF in the physiological response to burns.
- To analyze urinary EGF, urea, and creatinine levels in rats post-thermal injury.
Main Methods:
- A standardized thermal skin injury model was used on male rats (25% body surface area).
- Fluid resuscitation was administered.
- Urinary levels of EGF, urea, and creatinine were measured.
- Microscopic examination of skin lesions confirmed the absence of inflammatory complications.
Main Results:
- Body weight, urea, and creatinine excretion showed statistically significant differences compared to basal values.
- Urinary EGF excretion, when expressed as raw data, showed no significant difference from basal levels.
- A statistically significant increase in urinary EGF excretion (as a percentage of basal values) was observed within the first 7 days post-burn injury (P = 0.029).
Conclusions:
- Urinary EGF levels increase in response to burn injury in rats.
- EGF may be involved in the physiological response to thermal trauma.
- Urinary EGF can serve as a potential biomarker for burn injury.