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Related Experiment Videos

Antiseptic mouthrinse-induced microbial cell surface alterations

D Kubert1, M Rubin, M L Barnett

  • 1Warner-Lambert Company, Morris Plains, NJ 07950.

American Journal of Dentistry
|December 1, 1993
PubMed
Summary

Listerine Antiseptic mouthrinse causes significant changes to microbial cell surfaces. A brief 30-second exposure alters cell integrity, potentially leading to cell death in oral bacteria.

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Area of Science:

  • Microbiology
  • Oral Health
  • Microbial Morphology

Background:

  • Essential oil mouthrinses are widely used for oral hygiene.
  • Understanding the mechanism of action of antiseptic mouthrinses is crucial for oral health.
  • Previous studies have indicated that mouthrinses can alter surface-associated activities of microorganisms.

Purpose of the Study:

  • To investigate the effect of Listerine Antiseptic on the cell surface morphology of key oral microorganisms.
  • To determine if a short exposure time is sufficient to induce morphological changes.

Main Methods:

  • Scanning electron microscopy (SEM) was used to visualize microbial cell surfaces.
  • Five oral bacterial species (C. albicans, A. viscosus, S. sanguis, F. nucleatum, A. actinomycetemcomitans) were exposed to Listerine Antiseptic or buffer for 30 seconds.

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  • Standard fixation and dehydration protocols were employed for SEM analysis.

    • Main Results:

    • Listerine Antiseptic exposure induced distinct morphological alterations in microbial cell surfaces.
    • Changes observed included cell surface roughening, blebs, and other abnormalities, indicating a loss of cell surface integrity.
    • The extent of morphological damage varied among the different microbial species tested.

    Conclusions:

    • A brief 30-second exposure to Listerine Antiseptic causes significant morphological changes to oral microbial cell surfaces.
    • These morphological alterations suggest a disruption of cell integrity, which may contribute to cell death.
    • The findings provide a potential explanation for previously observed alterations in surface-associated microbial activities.