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Amphiphilic agarose-based adsorbents for chromatography. Comparative study of adsorption capacities and desorption
S Oscarsson1, D Angulo-Tatis, G Chaga
1Biochemical Separation Centre, Uppsala University, Sweden.
Journal of Chromatography. A
|January 6, 1995
Abstract:
A number of hydrophobic derivatives attached to cross-linked agarose were studied as protein adsorbents. Differences in the adsorption and desorption behaviour were determined as functions of type and concentration of selected salts. Whereas octyl- and phenyl-Sepharose adsorb serum albumin preferentially, pyridyl-S-agarose shows a much stronger preferential affinity for IgG in the presence of high concentrations of lyotropic salts, such as sulphates. In contrast to pyridyl-S-agarose, a large portion of proteins remained fixed to octyl- and phenyl-Sepharose after extensive washing with 1 M NaOH.