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Caprine pneumonia. II. Biochemical characterisation and serological identification of mycoplasmas
Abstract:
Thirty strains Mycoplasma isolated from pneumonic lungs of goats were chracterised biochemically and serologically. On the basis of biochemical characteristics, the strains were divided into three groups. (A) Eighteen strains which fermented glucose and mannose, digested serum and reduced tetrazolium, (B) 10 strains which reduced tetrazolium, formed "film and spots" and possessed phosphatase activity and (C) two strains which catabolised arginine only. Serologically group A strains were identified as M. mycoides subspecies, group B strains could not be fully identified but on the basis of immunofluorescence and biochemical reactions were put in the M. agalactiae subspecies; there are to be further investigated. Group C strains were identified as M. arginini.
Insights
Biochemical and serological characterization of Mycoplasma strains from goat pneumonia revealed three groups. Most strains were identified as Mycoplasma mycoides subspecies and Mycoplasma arginini, with some requiring further investigation.
Area of Science:
- Veterinary Microbiology
- Bacterial Pathogenesis
- Goat Respiratory Diseases
Background:
- Mycoplasma species are significant pathogens causing respiratory diseases in goats.
- Accurate identification of Mycoplasma strains is crucial for effective disease control and treatment strategies.
Purpose of the Study:
- To biochemically and serologically characterize Mycoplasma strains isolated from pneumonic goat lungs.
- To identify the Mycoplasma species involved in caprine pneumonia.
Main Methods:
- Biochemical characterization including fermentation of carbohydrates, serum digestion, tetrazolium reduction, film and spots formation, and phosphatase activity.
- Serological identification using immunofluorescence and comparison with known Mycoplasma subspecies.
- Isolation of Mycoplasma strains from pneumonic lung tissues of goats.
Main Results:
- Thirty Mycoplasma strains were categorized into three distinct biochemical groups.
- Group A (18 strains) identified as Mycoplasma mycoides subspecies.
- Group B (10 strains) tentatively identified as Mycoplasma agalactiae subspecies, requiring further investigation.
- Group C (2 strains) identified as Mycoplasma arginini.
Conclusions:
- The study successfully differentiated Mycoplasma strains from goat pneumonia based on biochemical and serological profiles.
- Mycoplasma mycoides subspecies and Mycoplasma arginini are implicated in caprine pneumonia.
- Further research is needed to fully characterize the strains provisionally identified as Mycoplasma agalactiae subspecies.