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Effects of transforming growth factor beta 1 on rainbow trout Oncorhynchus mykiss macrophage respiratory burst
S I Jang1, L J Hardie, C J Secombes
1Department of Zoology, University of Aberdeen, UK.
Abstract:
Natural bovine transforming growth factor beta 1 (TGF-beta 1) was found to have both enhancing and suppressing effects on the respiratory burst activity of rainbow trout, Oncorhynchus mykiss, head kidney macrophages. Incubation with TGF-beta 1 alone increased respiratory burst activity using doses from 0.05 to 0.2 ng/mL. However, incubation of activated macrophages with TGF-beta 1 decreased their respiratory burst activity in a dose-dependent manner. Coincubation of macrophages with activating signals (macrophage activating factor containing supernatants and tumor necrosis factor alpha) and TGF-beta 1 inhibited the ability of such signals to increase respiratory burst activity. Similarly, pretreatment of macrophages with TGF-beta 1 prior to treatment with activating signals resulted in a time and dose-dependent decrease in activity relative to cells not treated with TGF-beta 1. These suppressive effects were largest using higher doses of TGF-beta 1 (1 ng/mL). The conservation of TGF-beta s and their possible mode of action are discussed.