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Selective hydrolysis of tRNA by ethylenediamine bound to a DNA oligomer
1Department of Chemistry and Biotechnology, Faculty of Engineering, University of Tokyo.
Journal of Biochemistry
|October 1, 1994
Abstract:
A sequence-selective artificial ribonuclease was prepared by attaching ethylenediamine to the 5'-end of a DNA oligomer as the sequence-recognizing moiety. The hybrid, incorporating a 19-mer DNA which is complementary with the A44-A62 sequence of tRNA(Phe), hydrolyzed the tRNA selectively at the 3'-side of C63.