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Human arylhydrocarbon receptor: functional expression and chromosomal assignment to 7p21
M Ema1, N Matsushita, K Sogawa
1Department of Chemistry, Faculty of Science, Tohoku University, Sendai.
Journal of Biochemistry
|October 1, 1994
Summary
Researchers isolated human arylhydrocarbon receptor (AhR) cDNA, confirming its dioxin-binding and Arnt-dependent DNA-binding activities. The human AhR gene was localized to chromosome 7p21.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- The arylhydrocarbon receptor (AhR) is a crucial transcription factor involved in cellular responses to environmental stimuli.
- Understanding the human AhR is vital for comprehending toxicological pathways and gene regulation.
Purpose of the Study:
- To isolate and characterize the human arylhydrocarbon receptor (AhR) cDNA.
- To confirm the functional activities of the human AhR, including ligand binding and DNA binding.
- To determine the chromosomal location of the human AhR gene.
Main Methods:
- Isolation of human AhR cDNA using a mouse AhR cDNA probe from a human lung cDNA library.
- Expression of human AhR in COS-7 cells and in vitro translation systems.
- Assays for dioxin-binding and Arnt-dependent DNA-binding activities.
- Fluorescent in situ hybridization and DNA blot hybridization for gene localization.
Main Results:
- Human AhR cDNA was successfully isolated, with a nucleotide sequence identical to previously reported data.
- The expressed human AhR demonstrated specific dioxin-binding and Arnt-dependent DNA-binding capabilities.
- The human AhR gene was mapped to chromosome 7p21.
Conclusions:
- The study successfully isolated and functionally characterized human AhR.
- The findings confirm the conserved dioxin-binding and DNA-binding properties of human AhR.
- The precise chromosomal localization of the human AhR gene was established.