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Autoclaving of liposomes
1Institute of Experimental Cardiology, Moscow, Russia.
Journal of Microencapsulation
|November 1, 1994
Summary
Autoclaving liposomes after removing air prevents lipid oxidation and content leakage. Liposomes containing Intal remained stable and intact after a 15-minute sterilization cycle at 120°C.
Area of Science:
- Pharmaceutical Sciences
- Biotechnology
- Materials Science
Background:
- Liposomes are crucial drug delivery systems.
- Sterilization methods can compromise liposome integrity and efficacy.
- Autoclaving is a common sterilization technique but its effect on liposomes requires investigation.
Purpose of the Study:
- To evaluate the stability and oxidation of liposomes under autoclaving conditions.
- To determine if liposome integrity is maintained during steam sterilization.
- To assess the impact of autoclaving on entrapped drug content.
Main Methods:
- Liposome samples were prepared with entrapped Intal.
- Air was removed from liposome samples prior to autoclaving.
- Liposome stability was assessed by monitoring lipid oxidation using malonaldehyde bis(dimethyl acetate) as a control.
- Content leakage was measured.
- Liposome integrity was confirmed post-autoclaving (15 min at 120°C).
Main Results:
- Preliminary air removal prevented lipid oxidation during autoclaving.
- No significant lipid oxidation was detected.
- Liposome content leakage was negligible.
- Liposomes containing Intal remained completely intact after the autoclaving process.
Conclusions:
- Autoclaving is a viable sterilization method for liposomes when air is removed beforehand.
- Liposome integrity and drug content are preserved during autoclaving under these conditions.
- This finding supports the use of autoclaved liposomes in pharmaceutical applications.