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Chromosome mapping of Rfv3, a host resistance gene to Friend murine retrovirus
K J Hasenkrug1, A Valenzuela, V A Letts
1Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840.
Abstract:
Inoculation of adult mice with Friend virus complex usually induces rapid viremia and erythroleukemia, resulting in death in 1 to 3 months. In certain mouse strains, a single host gene, Rfv3, controls the ability to mount a virus-specific neutralizing antibody response which results in elimination of viremia. In this study, microsatellite markers were used to localize the Rfv3 gene to a 20-centimorgan region of mouse chromosome 15 unlinked to immunoglobulin loci, T-cell receptor loci, or the major histocompatibility complex. Potential candidate genes for Rfv3 are several genes expressed in cells of the immune system and previously mapped to the same region, including a T-cell antigen gene, Ly6, and three cytokine receptor genes, IL2rb, IL3rb1, and IL3rb2.
Insights
A specific gene, Rfv3, controls the immune response to Friend virus in mice. This study mapped Rfv3 to chromosome 15, identifying potential immune system candidate genes.
Area of Science:
- Immunology
- Genetics
- Virology
Background:
- Friend virus complex infection in mice typically leads to rapid viremia and erythroleukemia, causing mortality within 1-3 months.
- The Refv3 gene in certain mouse strains dictates the capacity for a virus-specific neutralizing antibody response, crucial for viremia elimination.
Purpose of the Study:
- To genetically map the Refv3 gene.
- To identify potential candidate genes for Refv3 function within the mapped region.
Main Methods:
- Utilized microsatellite markers for gene mapping.
- Analyzed mouse chromosome 15 for Refv3 localization.
- Compared Refv3 locus with known immunoglobulin, T-cell receptor, and major histocompatibility complex loci.
Main Results:
- Localized the Refv3 gene to a 20-centimorgan region on mouse chromosome 15.
- Confirmed Refv3 is unlinked to immunoglobulin, T-cell receptor, and MHC loci.
- Identified several immune-related candidate genes within the Refv3 region, including Ly6, IL2rb, IL3rb1, and IL3rb2.
Conclusions:
- The Refv3 gene, controlling Friend virus neutralization, is located on mouse chromosome 15.
- Potential candidate genes for Refv3 function include Ly6 and cytokine receptor genes (IL2rb, IL3rb1, IL3rb2).
- Further studies are warranted to confirm the identity and function of the Refv3 gene.