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Related Experiment Videos

Age-dependent changes in rat liver prenyltransferases

A Thelin1, M Runquist, J Ericsson

  • 1Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden.

Mechanisms of Ageing and Development
|October 20, 1994
PubMed
Summary

Enzymes in the mevalonate pathway show varied activity during rat development. Cholesterol levels remain stable, while dolichol concentrations increase, highlighting dynamic lipid regulation in early life.

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Area of Science:

  • Biochemistry
  • Cellular Biology
  • Developmental Biology

Background:

  • Mevalonate pathway lipids, including cholesterol, ubiquinone, and dolichol, are vital for cellular functions.
  • Enzymes within this pathway are tightly regulated, especially during developmental stages.
  • Lipid levels and enzyme activities can fluctuate significantly during organism development.

Purpose of the Study:

  • To investigate the developmental changes in prenyltransferase activities in rat liver cytosol.
  • To correlate enzyme activity with protein levels for key enzymes in the mevalonate pathway.
  • To examine the dynamic changes in specific lipid concentrations during postnatal rat development.

Main Methods:

  • Assay of farnesyl pyrophosphate synthase, cis-prenyltransferase, and squalene synthase activities in rat liver cytosol.

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  • Quantification of synthase protein levels at various postnatal time points.
  • Measurement of cholesterol and dolichol concentrations throughout the study period.

    • Main Results:

    • Farnesyl pyrophosphate synthase activity decreased post-birth, while its protein level showed two peaks.
    • cis-Prenyltransferase activity increased up to day 54, then decreased, with low sustained levels thereafter.
    • Squalene synthase activity was high at birth, decreasing significantly within 100 days.
    • Cholesterol levels remained constant, whereas dolichol concentration showed a continuous increase during development.

    Conclusions:

    • Enzyme activities of prenyltransferases in rat liver cytosol undergo distinct developmental regulation.
    • Postnatal development involves complex, differential regulation of mevalonate pathway enzymes.
    • Cholesterol and dolichol levels exhibit distinct regulatory patterns compared to the enzymatic activities studied.