Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Genetically engineered in vitro systems for biotransformation studies

J Doehmer¹, W A Schmalix, H Greim

¹Institut für Toxikologie und Umwelthygiene, Technischen Universität München, Germany.

Methods and Findings in Experimental and Clinical Pharmacology

|September 1, 1994

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Necessity of Developing New Criteria for Carcinogen Classification of Fibers by the Mak Commission.

Inhalation toxicology·2015

Same author

The Use of Long-term Hepatocyte Cultures for Detecting Induction of Drug Metabolising Enzymes: The Current Status.

Alternatives to laboratory animals : ATLA·2014

Same author

A toxicological and dermatological assessment of alkyl cyclic ketones when used as fragrance ingredients. RIFM Expert Panel.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2013

Same author

A toxicologic and dermatologic assessment of cyclopentanones and cyclopentenones when used as fragrance ingredients.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2012

Same author

A toxicological and dermatological assessment of aryl alkyl alcohol simple acid ester derivatives when used as fragrance ingredients.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2012

Same author

A toxicological and dermatological assessment of aryl alkyl alcohols when used as fragrance ingredients.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2011

Same journal

[Proceedings of the XVIII TASPE Seminar, Granada, Spain, 4-5 February 2010].

Methods and findings in experimental and clinical pharmacology·2011

Same journal

[Treatment algorithm for gastrointestinal graft-versus-host disease].

Methods and findings in experimental and clinical pharmacology·2011

Same journal

[Treatment of chronic graft-versus-host disease with protein tyrosine kinase inhibitors].

Methods and findings in experimental and clinical pharmacology·2011

Same journal

[Classification of chronic graft-versus-host disease].

Methods and findings in experimental and clinical pharmacology·2011

Same journal

[Pegfilgrastim in hematopoietic stem cell transplantation].

Methods and findings in experimental and clinical pharmacology·2011

Same journal

[Allogeneic transplantation in multiple myeloma patients: results and recommendations in February 2010].

Methods and findings in experimental and clinical pharmacology·2011

See all related articles

Genetically engineered cell systems expressing cytochrome P450 enzymes enable the study of drug and pollutant metabolism. These systems are crucial for assessing metabolism-dependent pharmacological and toxicological effects.

Area of Science:

Biochemistry
Pharmacology
Toxicology

Background:

Cytochrome P450 enzymes are critical for metabolizing xenobiotics like drugs and environmental pollutants.
Understanding xenobiotic metabolism is essential for drug development and environmental safety assessments.

Purpose of the Study:

To develop and define genetically engineered cell systems for studying cytochrome P450-mediated metabolism.
To enable the assessment of metabolism-dependent pharmacological and toxicological effects.

Main Methods:

Genetically engineering cell systems by cloning cDNAs encoding cytochrome P450 and other relevant enzymes.
Utilizing heterologous expression systems in bacterial, yeast, and mammalian cells.
Defining genetically engineered cell systems based on specific cDNA enzyme function.

Related Experiment Videos

Main Results:

Established functional cell systems for cytochrome P450-mediated xenobiotic metabolism.
Demonstrated the utility of these systems for evaluating metabolism-dependent effects.

Conclusions:

Genetically engineered cell systems provide a powerful tool for understanding xenobiotic metabolism.
These systems facilitate the assessment of drug and pollutant interactions with biological systems.