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A new method for factor VII deficient substrate preparation and coagulation studies in malaria
S Rojanasthien1, V Surakamollert, P Isarangkura
1Hematology Division, Ramathibodi Hospital, Faculty of Medicine, Mahidol University, Bangkok, Thailand.
Summary
A new, cost-effective method for preparing factor VII deficient substrate was developed. This method aids in studying coagulation and fibrinolysis abnormalities in malaria patients, particularly P. falciparum infections.
Area of Science:
- Hematology
- Clinical Chemistry
- Parasitology
Background:
- Routine factor VII assays are costly.
- A simplified, affordable substrate is needed for coagulation laboratories.
Purpose of the Study:
- To develop a cost-effective factor VII deficient substrate using DEAE-cellulose chromatography.
- To investigate coagulation and fibrinolysis abnormalities in malaria patients.
Main Methods:
- DEAE-cellulose chromatography for substrate preparation.
- Correlation analysis with commercial substrates.
- Coagulation and fibrinolysis testing in 250 malaria patients (P. vivax and P. falciparum).
Main Results:
- The prepared substrate showed high correlation with commercial ones and was significantly cheaper.
- Plasmodium falciparum infections correlated with prolonged prothrombin time (PT) and activated partial thromboplastin time (aPTT).
- Increased plasmin activity was observed in P. falciparum patients with high parasitemia.
Conclusions:
- The developed DEAE-cellulose chromatography method provides an economical alternative for factor VII deficient substrate preparation.
- Coagulation defects, particularly in P. falciparum malaria, are linked to parasitemia levels and liver involvement.
- Plasmin activity elevation in P. falciparum malaria suggests a role in disease pathogenesis.
