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A new method of paternity testing for dogs, based on microsatellite sequences
I Zajc1, C Mellersh, E P Kelly
1Department of Biochemistry, University of Leicester.
The Veterinary Record
|December 3, 1994
Summary
Canine microsatellites, despite lower variability, are effective for dog paternity testing. This DNA method is simpler and requires less blood than traditional fingerprinting, aiding in pedigree breed assignments.
Area of Science:
- Genetics
- Molecular Biology
- Forensic Science
Background:
- Microsatellite DNA sequences are polymorphic and found in mammals.
- Lower variability in microsatellites and canine inbreeding raised doubts about their use in paternity disputes.
- Canine minisatellites (DNA fingerprinting) have been used but are more complex.
Purpose of the Study:
- To evaluate the effectiveness of canine microsatellites for paternity testing in pedigree dogs.
- To demonstrate that microsatellites provide an adequate basis for assigning paternity.
- To present a more straightforward system compared to minisatellite-based methods.
Main Methods:
- Analysis of microsatellite DNA sequences in pedigree dog populations.
- Comparison of microsatellite analysis with established canine minisatellite DNA fingerprinting techniques.
- Development of a simplified protocol for microsatellite analysis.
Main Results:
- Canine microsatellites demonstrate sufficient variability for reliable paternity assignment in pedigree breeds.
- The microsatellite system is more straightforward to perform and interpret than minisatellite DNA fingerprinting.
- The method requires a minimal blood sample volume (0.1 ml).
Conclusions:
- Canine microsatellites are a viable and effective tool for dog paternity testing.
- The presented microsatellite system offers a simpler, more efficient alternative to DNA fingerprinting for canine parentage verification.
- This method supports genetic management and dispute resolution in pedigree dog breeding.