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Related Experiment Videos

Chimeric macaque/human Fab molecules neutralize simian immunodeficiency virus

A Samuelsson1, F Chiodi, P Ohman

  • 1Karolinska Institute, Department of Medicine, Rolf Luft's Center, Karolinska Hospital, Stockholm, Sweden.

Virology
|March 10, 1995
PubMed
Summary
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Researchers developed novel simian immunodeficiency virus (SIV) neutralizing antibody fragments. These potent reagents, derived from macaques and humans, show promise for preventing and treating lentivirus infections.

Area of Science:

  • Immunology
  • Virology
  • Biotechnology

Background:

  • Simian immunodeficiency virus (SIV) infection poses a significant challenge in non-human primate models.
  • Developing effective countermeasures against SIV is crucial for understanding and combating lentivirus infections.

Purpose of the Study:

  • To generate and characterize novel SIV-neutralizing recombinant Fab fragments.
  • To explore the potential of these fragments for prophylactic and therapeutic applications against lentivirus infections.

Main Methods:

  • Utilized a combinatorial antibody library approach using phage display.
  • Constructed chimeric antibody fragments from macaque and human immunoglobulin chains.
  • Screened fragments against purified SIVsm surface glycoprotein (gp148).

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Main Results:

  • Identified twelve chimeric Fab fragments that reacted with SIVsm gp148.
  • Six fragments demonstrated potent neutralizing activity against SIVsm at low concentrations (0.01-0.1 µg/ml).
  • Nucleic acid sequencing confirmed that all neutralizing fragments were clonally unrelated.

Conclusions:

  • Developed potent, clonally distinct SIV-neutralizing Fab fragments.
  • These fragments represent promising candidates for immune intervention in SIV-infected macaques.
  • Further studies will map neutralizing determinants of SIV using these reagents.