Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Marrow regeneration after mechanical depletion

G Brecher, J H Tjio, W W Smith

    Blood
    |November 1, 1976
    PubMed
    Summary

    Marrow regeneration originates from local stem cells, not a common precursor. Hemopoietic stem cells are not found in Haversian canals, and bone formation isn't required for marrow regeneration.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Lake Keuka Medical and Surgical Association.

    Buffalo medical journal·2023
    Same author

    Simple locking of infrared and ultraviolet diode lasers to a visible laser using a LabVIEW proportional-integral-derivative controller on a Fabry-Perot signal.

    The Review of scientific instruments·2016
    Same author

    Dynamics of hemopoietic proliferation in man and mice studied by H3-thymidine incorporation into DNA.

    Progress in nuclear energy. Series 6 Biological sciences·2014
    Same author

    Off-resonance energy absorption in a linear Paul trap due to mass selective resonant quenching.

    The Review of scientific instruments·2013
    Same author

    A novel angiogenesis inhibitor impairs lovo cell survival via targeting against human VEGFR and its signaling pathway of phosphorylation.

    Cell death & disease·2012
    Same author

    Synphilin-1 alters metabolic homeostasis in a novel Drosophila obesity model.

    International journal of obesity (2005)·2012

    Area of Science:

    • Hematology
    • Stem Cell Biology
    • Bone Marrow Biology

    Background:

    • Understanding marrow regeneration is crucial for regenerative medicine and treating hematological disorders.
    • Previous studies suggested diverse origins for marrow-repopulating cells, requiring further investigation.

    Purpose of the Study:

    • To elucidate the origin of hematopoietic stem cells during marrow regeneration after mechanical depletion.
    • To investigate the role of local stem cell remnants versus a common precursor in marrow repopulation.

    Main Methods:

    • Utilized mouse chimeras to track cell origins after mechanical marrow depletion.
    • Employed a modified in vivo evacuation technique to deplete bone marrow in transplanted femurs.
    • Analyzed hemopoiesis and osteogenesis in depleted and non-depleted control groups.

    Main Results:

    • Results supported the local origin of stem cells from residual marrow, excluding a common precursor for bone and hematopoietic lineages.
    • Failure of hemopoietic regeneration in femurs depleted via in vivo evacuation excluded Haversian canals as a source of hemopoietic stem cells.
    • Significant hemopoiesis occurred with minimal bone formation in necrotic controls, indicating osteogenesis is not essential for hematopoietic regeneration.

    Conclusions:

    • Hematopoietic stem cells for marrow regeneration arise from local remnants, not a shared precursor.
    • Haversian canals do not harbor functional hemopoietic stem cells.
    • Osteogenesis is not a prerequisite for hemopoiesis during marrow regeneration.

    Related Experiment Videos