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Related Experiment Videos

Chitosan as a matrix for mammalian cell encapsulation

B A Zielinski1, P Aebischer

  • 1Section of Artificial Organs, Biomaterials and Cellular Technology, Brown University, Providence RI.

Biomaterials
|October 1, 1994
PubMed
Summary

Supportive matrices like chitosan enhance cell viability within microcapsules and macrocapsules. Precipitated chitosan within acrylonitrile-vinylchloride copolymer macrocapsules effectively supported fibroblast and PC12 cell growth and function.

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Tissue Engineering

Background:

  • Encapsulating cells within semi-permeable membranes can improve viability and proliferation.
  • Supportive matrices are crucial for cell growth within encapsulated systems.
  • Investigating different matrices is key to optimizing cell encapsulation.

Purpose of the Study:

  • To evaluate the influence of anionic sodium alginate and cationic chitosan as supportive matrices on cell growth.
  • To compare the efficacy of microcapsules and macrocapsules for cell encapsulation.
  • To assess the viability and function of encapsulated PC12 and fibroblast cell lines.

Main Methods:

  • Cells (PC12, R208F, R208N.8) were encapsulated in sodium alginate microcapsules, crosslinked chitosan microcapsules, and PAN/PVC macrocapsules with precipitated chitosan.

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  • Microcapsules were cultured for 2 weeks, and macrocapsules for 4 weeks in vitro.
  • Cell proliferation, viability, catecholamine release (PC12), and nerve growth factor (NGF) release (R208N.8) were assessed.

    • Main Results:

    • PC12 and R208F cells proliferated in alginate microcapsules; PC12 cells released catecholamines.
    • R208N.8 cells showed poor support in alginate, with viable cells protruding from the matrix.
    • All cell lines grew poorly in crosslinked chitosan microcapsules but showed excellent viability in PAN/PVC macrocapsules with precipitated chitosan.

    Conclusions:

    • Precipitated chitosan as a matrix within PAN/PVC macrocapsules supports fibroblast attachment, spreading, and viability.
    • The choice of matrix and encapsulation system significantly impacts cell growth and function.
    • Cationic hydrogels like chitosan show promise as supportive matrices for cell encapsulation technologies.