Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene activation studied by immunological methods

H S Huitfeldt1, A Heyden, E Skarpen

  • 1Department of Environmental Medicine, National Institute of Public Health, Oslo, Norway.

Environmental Health Perspectives
|October 1, 1994
PubMed
Summary

Investigating gene activation requires studying DNA, mRNA, and protein alterations using combined in situ and biochemical methods. These techniques help identify xenobiotic-induced changes in cellular processes and differentiation markers.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification and evaluation of Pharmacological enhancers of the factor VII p.Q160R variant.

Scientific reports·2025
Same author

Low levels of antibodies for the oral bacterium Tannerella forsythia predict cardiovascular disease mortality in men with myocardial infarction: A prospective cohort study.

Medical hypotheses·2020
Same author

Enhanced targeting of triple-negative breast carcinoma and malignant melanoma by photochemical internalization of CSPG4-targeting immunotoxins.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology·2018
Same author

Mechanisms linked to differences in the mutagenic potential of 1,3-dinitropyrene and 1,8-dinitropyrene.

Toxicology reports·2017
Same author

Characterization and pro-inflammatory responses of spore and hyphae samples from various mold species.

Indoor air·2017
Same author

Air pollution-related metals induce differential cytokine responses in bronchial epithelial cells.

Toxicology in vitro : an international journal published in association with BIBRA·2016

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Toxicology

Background:

  • Gene activation studies can occur at transcriptional, translational, or phenotypical levels.
  • Studying xenobiotic effects requires accounting for cellular heterogeneity within organs.
  • Immunological probes are crucial for detecting xenobiotic-induced alterations in DNA, mRNA, and proteins.

Purpose of the Study:

  • To explore methods for studying gene activation at multiple levels.
  • To investigate the use of immunological probes for xenobiotic-induced alterations.
  • To examine the impact of xenobiotics on DNA modifications and cellular differentiation.

Main Methods:

  • Utilized in situ and biochemical techniques for studying gene activation.
  • Employed immunological probes to identify and localize proteins and modified DNA.

Related Experiment Videos

  • Combined 2-acetylaminofluorene (AAF) adduct identification with bromodeoxyuridine (BrdU) pulse labeling.
  • Applied BrdU pulse chase experiments with double immunofluorescence to study keratin expression in mouse skin.

    • Main Results:

    • Developed techniques to detect reduced DNA adduct concentrations in proliferating cells and preneoplastic foci in AAF-fed rats.
    • Demonstrated that carcinogen-induced DNA modifications are linked to altered gene activation and neoplasia.
    • Showed that xenobiotics can affect cellular differentiation, using keratin K1 and K10 as markers.
    • Revealed sequential keratin expression and cell migration patterns following DNA synthesis and in response to tumor promoters like TPA.

    Conclusions:

    • Combined in situ and biochemical methods, particularly immunological probes, are essential for comprehensive gene activation studies.
    • Xenobiotics can induce DNA modifications and alter cellular differentiation, impacting gene activation pathways.
    • Understanding these molecular mechanisms is critical for assessing the toxicological and carcinogenic potential of xenobiotics.