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Inhibition of cellular protein secretion by poliovirus proteins 2B and 3A

J R Doedens1, K Kirkegaard

  • 1Department of Molecular, Cellular and Developmental Biology, Howard Hughes Medical Institute, University of Colorado, Boulder 80309-0347.

The EMBO Journal
|March 1, 1995
PubMed

Insights

Poliovirus infection disrupts host cell protein transport early in the viral life cycle. Viral proteins 2B and 3A individually block transport from the endoplasmic reticulum, impacting cellular function.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Poliovirus RNA replication occurs on cytoplasmic vesicles.
  • These vesicles are hypothesized to originate from the host cell's secretory pathway.

Purpose of the Study:

  • To investigate the impact of poliovirus infection on protein transport through the secretory pathway.
  • To identify viral components responsible for transport inhibition.

Main Methods:

  • Examined protein transport in poliovirus-infected cells.
  • Assessed the roles of viral RNA replication and host translation inhibition.
  • Investigated the effects of individual viral proteins (2B, 3A) on protein transport.
  • Determined the intracellular localization of secreted proteins in the presence of viral proteins.

Main Results:

  • Poliovirus infection inhibits transport of plasma membrane and secretory proteins early in infection.
  • Transport inhibition occurs independently of viral RNA replication and host translation inhibition.
  • Viral proteins 2B and 3A are individually sufficient to inhibit protein transport.
  • Viral protein 3A causes secreted proteins to accumulate in the endoplasmic reticulum.

Conclusions:

  • Poliovirus infection significantly disrupts host cell secretory pathway function.
  • Viral proteins 2B and 3A play critical roles in blocking protein transport.
  • Protein 3A appears to directly inhibit transport from the endoplasmic reticulum to the Golgi apparatus.

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