Related Experiment Video
Updated: Aug 3, 2026
09:04
Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
Novel aromatase transcripts from bovine placenta contain repeated sequence motifs
1Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere, Dummerstorf, Germany.
Gene
|March 10, 1995
Summary
Researchers isolated and analyzed the bovine aromatase cytochrome P-450 transcript (bCyp19). The study revealed significant sequence homology to other species and identified unique features in the bovine 3'-untranslated region, including repetitive elements.
Area of Science:
- Biochemistry
- Genetics
- Molecular Biology
Background:
- Aromatase cytochrome P-450 (Aro) is the key enzyme in estrogen biosynthesis.
- Understanding gene structure and regulation is crucial for reproductive biology.
Purpose of the Study:
- To isolate and perform comparative sequence analysis of the bovine aromatase cytochrome P-450 transcript (bCyp19).
- To elucidate the structural characteristics of the bovine bCyp19 gene, particularly its untranslated regions.
Main Methods:
- Isolation of bCyp19 cDNA clones from a placental lambda gt10 cDNA library.
- Sequencing of overlapping cDNA clones to determine the full transcript length.
- Comparative sequence analysis against human, rat, and mouse aromatase genes.
Main Results:
- A 5180 bp sequence of bCyp19 was derived, featuring two adjacent polyadenylation sites.
- The bovine open reading frame (ORF) shows high homology (87-78%) to mammalian counterparts.
- The 3'-untranslated region (UTR) is significantly longer than the human equivalent, containing bovine repeat elements and a novel 5'-UTR sequence.
Conclusions:
- Bovine bCyp19 is likely transcribed into multiple mRNA species.
- The extended 3'-UTR of bCyp19 is attributed to insertions of repetitive sequences and contains elements found only on the human genomic level.
- A functional polyadenylation signal in the bovine transcript originates from a downstream repeat element.
More Related Videos
09:11Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration
Published on: September 27, 2022
07:03A Modified Co-Culture System for Understanding Granulosa-Theca Cell Interactions in the Bovine Ovary
Published on: September 19, 2025
Related Concept Videos
03:18Non-LTR Retrotransposons
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
02:02Cis-regulatory Sequences
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
02:28Leaky Scanning
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
03:21RNA-seq
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
02:24Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
02:02Cis-regulatory Sequences
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...