Related Experiment Videos
Distribution and characterization of specific cellular binding proteins for bone morphogenetic protein-2
S Iwasaki1, N Tsuruoka, A Hattori
1Department of Biology, Gifu Pharmaceutical University, Japan.
The Journal of Biological Chemistry
|March 10, 1995
Summary
Bone morphogenetic proteins (BMPs) are multifunctional proteins. BMP-2 binding sites are widespread on non-hematopoietic cells, suggesting diverse physiological roles beyond bone and cartilage formation.
Area of Science:
- Biochemistry and Molecular Biology
- Cell Biology
- Developmental Biology
Background:
- Bone morphogenetic proteins (BMPs) are known for inducing cartilage and bone formation.
- BMPs are structurally related to transforming growth factor-beta (TGF-β), activins, and inhibins.
- Understanding BMPs' physiological functions and mechanisms requires identifying their cellular targets.
Purpose of the Study:
- To investigate the distribution of specific cellular binding proteins for BMP-2.
- To identify potential target cells for BMP-2 action.
Main Methods:
- Examined BMP-2 binding to various cell types using radiolabeled BMP-2.
- Utilized affinity cross-linking to identify BMP-2 binding components.
- Assessed competition for BMP-2 binding with other growth factors.
Main Results:
- High-affinity BMP-2 binding sites were found ubiquitously on major non-hematopoietic cells, including osteoblastic cells, fibroblasts, keratinocytes, and various tumor cells.
- BMP-2 binding sites were absent on vascular endothelial cells and hematopoietic cells.
- Affinity cross-linking revealed common molecular mass components (170, 105, 90, 80, 70 kDa) in fibroblast cell lines.
Conclusions:
- Major cell types, excluding hematopoietic and vascular endothelial cells, are potential targets for BMP-2.
- BMP-2 exhibits a broader distribution of binding sites than initially suggested by its role in bone formation.
- These findings provide a foundation for further research into BMP-2's diverse physiological roles.