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[Regulation of transcription of the interleukin-2 gene in B-lymphocytes]

A Mouzaki1, R H Zubler

  • 1Département de médecine, Hôpital cantonal universitaire de Genève.

Schweizerische Medizinische Wochenschrift
|March 4, 1995
PubMed

Since most B cell clones immortalized with EBV virus can be induced to produce interleukin-2, a typical T cell cytokine, we studied the role of different elements of the IL-2 promoter in such clones by transfection. It was found, in particular, that the element TCEd, which binds the transcription factor NF-kB, is very active in all three B clones tested. This element has no activity in T cells of the Jurkat line. The NFATd element, which binds the transcription factor NFAT-1 and is very active in T cells, is only weakly active in one B clone and not at all in another. Different elements thus contribute to IL-2 promoter activity in different cells.

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