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V3 sequences in primary HIV-1 infection
I M Antonioli1, C Baumberger, S Yerly
1Department of Medicine, Geneva University Hospital, Switzerland.
AIDS (London, England)
|January 1, 1995
Summary
HIV-1 genomic RNA and proviral DNA sequences are identical in primary HIV infection (PHI). No distinct V3 loop patterns differentiate PHI from advanced HIV-1 infection, though some isolates show lymphotropism markers.
Area of Science:
- Virology
- Molecular Biology
- Immunology
Background:
- Primary HIV-1 infection (PHI) is a critical window for understanding viral dynamics.
- The V3 loop of HIV-1's envelope protein is crucial for viral entry and tropism.
- Comparing early and advanced infection stages reveals viral evolution patterns.
Purpose of the Study:
- To sequence the V3 loop of HIV-1 genomic RNA and proviral DNA in PHI patients.
- To compare these sequences with those from patients with advanced HIV-1 infection.
- To identify potential sequence characteristics associated with PHI.
Main Methods:
- Collected sera and PBMCs from 24 PHI patients.
- Determined V3 loop sequences using direct solid-phase sequencing of PCR products.
- Analyzed sequence homology and amino acid characteristics.
Main Results:
- 100% homology observed between genomic RNA and proviral DNA V3 sequences in PHI patients.
- Three isolates exhibited the lymphotropism-associated QRGPGR signature in the V3 loop.
- No significant differences in V3 loop amino acid distribution between PHI and advanced HIV-1 infection (B subtype).
Conclusions:
- HIV-1 genomic RNA and proviral DNA V3 sequences are identical in PHI.
- The QRGPGR signature was found in a subset of PHI isolates.
- No specific V3 loop sequence pattern characterizes primary HIV-1 infection.