Recent progress in the electrochemistry of c-type cytochromes

C-type cytochromes are classified into two main groups: i) cytochromes which give fast electrochemical responses at the conventional electrodes in the absence of any promoter (eg multi-heme cytochromes c3); ii) cytochromes which need the presence of promoters or the use of modified electrodes to exhibit fast electrochemical responses (eg one-heme mitochondrial cytochrome c). In the latter case, careful design of electrode surface and composition of the solution are required for the attainment of rapid and reversible electron-exchange reactions. Some general considerations are given on the 'electrochemical model'. In particular, binding interactions between the electrode and the protein can take place in a similar manner to that occurring between physiological partner proteins. Electrochemistry when coupled to other physical techniques can give more complete insights in the relationship between the redox properties, structure and function of c-type cytochromes. In particular, in the case of polyheme cytochromes, promising results are expected from the study of site-directed mutagenesis-modified cytochrome c3.