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A new method for direct analysis of polymerase chain reaction-amplified human papillomavirus using DNA enzyme
F Sangiuolo1, L De Santis, A Cavicchini
1Department of Human Genetics, Tor Vergata University of Rome, Italy.
International Journal of Clinical & Laboratory Research
|January 1, 1994
Abstract:
We describe use of a polymerase chain reaction in conjunction with a DNA enzyme immunoassay for the simultaneous detection of multiple types of human papillomavirus in a single microtiter plate. The method is specific, sensitive, rapid, and capable of detecting human papillomavirus-6, -11, -16, -18, and -33 in cervical biopsy specimens.