Assessment of proliferative activity in type II pneumocytes after inhalation of NO2 by AgNOR-analysis

Adult male Sprague Dawley rats were exposed to 0.8, 5 and 10 ppm NO, alternatively for 1 or 3 days. After exposure the proliferative activity of the airway epithelium was assessed by means of BrdU incorporation and AgNOR-analysis at three different airway levels--in the bronchial, the bronchiolar epithelium and in Type 2 cells. The BrdU-labeling index (BrdU-LI), and AgNOR-number (AgNOR-N) were used to quantify the epithelial proliferation. Cytologic specimens of Type II cells showed a significant increase of the AgNOR-number and BrdU-LI after exposure to 5 ppm NO2 for 3 days and 10 ppm for 1 and 3 days. In the bronchiolar epithelium both, the AgNOR-number and BrdU-LI were significantly elevated in all the exposure groups. In the bronchial epithelium a significant response of the AgNOR-number was found after exposure to 10 ppm for 3 days. The correlation between AgNOR-number and BrdU-LI was 0.78 (p < 0.005).