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[The mechanism of action of permethrin on house dust mites]

O Iu Eremina, G N Stepanova

    Izvestiia Akademii Nauk. Seriia Biologicheskaia
    |January 1, 1995
    PubMed

    Insights

    Home dust mites, Dermatophagoides farinae and D. pteronyssinus, exhibit resistance to permethrin. Detoxification involves monoxygenases and esterases, with qualitative differences found in their enzyme complexes between species.

    Area of Science:

    • Entomology
    • Toxicology
    • Biochemistry

    Background:

    • Home dust mites are significant allergens.
    • Permethrin is a common acaricide used for dust mite control.
    • Understanding resistance mechanisms is crucial for effective pest management.

    Purpose of the Study:

    • To investigate the mechanism of permethrin action on Dermatophagoides farinae and Dermatophagoides pteronyssinus.
    • To identify factors contributing to permethrin resistance in these dust mite species.
    • To elucidate the role of specific enzymes in permethrin detoxification.

    Main Methods:

    • Toxicity evaluations under various conditions.
    • Investigation of cuticle permeability.
    • Analysis of monoxygenase and esterase activity in permethrin detoxification.
    • Electrophoresis in polyacrylamide gel (PAAG) to examine esterase enzyme complexes.
    • Identification of esterase activity zones using 2-naphthyl acetate (2-NA) and 4-nitrophenyl acetate (ATI) substrates.

    Main Results:

    • Both Dermatophagoides farinae and Dermatophagoides pteronyssinus demonstrated resistance to permethrin.
    • Monoxygenases and esterases were identified as key enzymes in the permethrin detoxification pathway.
    • Qualitative differences were observed in the esterase enzyme complexes between the two dust mite species.
    • Cuticle permeability was investigated as a potential factor in resistance.

    Conclusions:

    • Permethrin resistance in home dust mites is mediated by enzymatic detoxification, primarily involving monoxygenases and esterases.
    • Distinct qualitative profiles of esterase complexes contribute to differential resistance mechanisms between Dermatophagoides species.
    • Further research into these enzymatic differences could inform novel strategies for dust mite control.

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