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Development of preparing small-amount samples for transmission electron microscopy

Y Kaji¹, K Kawamoto, S Wakisaka

¹Department of Neurosurgery, Miyazaki Medical College.

Noshuyo Byori = Brain Tumor Pathology

|January 1, 1994

Summary

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Researchers determined the minimum cell quantity for transmission electron microscopy (TEM). Over 1 x 10^3 cells are sufficient for TEM, preserving cellular structures for detailed analysis.

Area of Science:

Cell Biology
Microscopy Techniques

Background:

Transmission electron microscopy (TEM) requires specific sample preparation.
Determining the minimum sample size is crucial for efficient analysis.

Purpose of the Study:

To establish the minimum number of cells required for successful TEM examination.
To evaluate the preservation of cellular structures in small-amount samples.

Main Methods:

Cultured cells (KMU-100) were diluted to various concentrations in phosphate-buffered saline (PBS).
Cells were collected via centrifugation, fixed, dehydrated, and embedded in Epon 812.
Samples were sectioned, stained, and analyzed using TEM.

Main Results:

A minimum of 1 x 10^3 cells were observable using TEM.

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Despite procedural damage, cellular microscopic structures were relatively maintained compared to cell monolayers.

Conclusions:

TEM can effectively analyze small cell samples, requiring over 1 x 10^3 cells.
This method allows for the examination of cellular ultrastructure from limited cell populations.