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Solid-phase extraction and reversed-phase high-performance liquid chromatographic determination of sulphur mustard in
R S Dangi1, K Jeevaratnam, K Sugendran
1Defence Research and Development Establishment, Gwalior, India.
Journal of Chromatography. B, Biomedical Applications
|November 18, 1994
Abstract:
A reversed-phase high-performance liquid chromatographic method is reported for the analysis of sulphur mustard in blood with the aid of solid-phase extraction sample preparation. Sulphur mustard is extracted from blood samples (both in vitro and in vivo) of rats with a solution of 0.05 M sodium dodecyl sulphate and pre-concentrated over Sep-Pak C18 cartridges pre-coated with Tween-20. A Polygosil C18 column is used with acetonitrile-water (52:48, v/v) as mobile phase for separation and sulphur mustard was detected at 200 nm.