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Characterization of Streptococcus suis capsular type 2 haemolysin

M G Gottschalk1, S Lacouture, J D Dubreuil

  • 1Groupe de Recherche sur les Maladies Infectieuses du Porc (GREMIP), Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada.

Microbiology (Reading, England)
|January 1, 1995
PubMed
Summary

Streptococcus suis capsular type 2 produces a novel 65 kDa haemolysin. This cholesterol-binding cytolytic toxin

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Area of Science:

  • Microbiology
  • Toxicology
  • Biochemistry

Background:

  • Streptococcus suis is a significant pathogen in pigs and can cause human infections.
  • Haemolysins are virulence factors produced by various bacteria, contributing to pathogenesis.
  • The characterization of virulence factors in S. suis is crucial for understanding disease mechanisms.

Purpose of the Study:

  • To investigate the production and characteristics of a haemolysin from Streptococcus suis capsular type 2.
  • To determine the susceptibility of different erythrocytes to the S. suis haemolysin.
  • To classify the haemolysin based on its biochemical and functional properties.

Main Methods:

  • Investigation of haemolysin production during bacterial growth phases.
  • Purification of the haemolysin using affinity chromatography (thiopropyl-Sepharose 6B).

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  • Characterization of the haemolysin's molecular mass, sensitivity to oxygen/reducing agents, and inhibition by cholesterol.

    • Main Results:

    • A 65 kDa extracellular haemolysin was purified from S. suis capsular type 2.
    • Human group O erythrocytes were most susceptible; rabbit erythrocytes were least susceptible.
    • The haemolysin shares characteristics with cholesterol-binding cytolytic toxins and is inhibited by anti-streptolysin antibodies.

    Conclusions:

    • Streptococcus suis capsular type 2 produces a unique haemolysin belonging to the cholesterol-binding cytolytic toxin family.
    • The haemolysin's activity was demonstrated against various red blood cells, with differential susceptibility.
    • Further investigation is needed to elucidate the role of this haemolysin in S. suis pathogenesis.