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Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
Published on: July 5, 2018
Ly-49A, a receptor for H-2Dd, has a functional carbohydrate recognition domain
B F Daniels1, M C Nakamura, S D Rosen
1Department of Medicine, University of California, San Francisco 94143.
Abstract:
Ly-49A+ murine natural killer (NK) cells cannot lyse target cells that express H-2Dd. We demonstrate a functional requirement for carbohydrate recognition by Ly-49A. Treatment of H-2Dd+ target cells with tunicamycin prevents their binding to Ly-49A+ cells and renders them susceptible to lysis by Ly-49A+ NK cells. Fucoidan, a sulfated polysaccharide, binds to Ly-49A in a calcium-dependent manner, and this binding is inhibited by monosaccharides, particularly sulfated hexoses. The inactivation of Ly-49A+ NK cells by H-2Dd+ target cells is reversed in the presence of glucose 6-SO4. These results indicate that Ly-49A has a functional carbohydrate recognition domain and that target expression of carbohydrates alters their susceptibility to natural killing.
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