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Ontogeny of calbindin-D28K and calretinin in developing chick kidney
L S Daneo1, G Corvetti, G L Panattoni
1Department of Human Anatomy and Physiology, University of Turin, Italy.
Insights
This study reveals the early development of calcium-binding proteins calbindin-D28k and calretinin in chick kidneys. Their presence in developing tubules suggests a role in calcium regulation during kidney formation.
Area of Science:
- Developmental Biology
- Renal Physiology
- Molecular Biology
Background:
- Calcium-binding proteins play crucial roles in cellular functions.
- Understanding the ontogeny of these proteins in the kidney is essential for comprehending renal development and function.
- Calbindin-D28k and calretinin are key calcium-binding proteins with incompletely understood roles in kidney development.
Purpose of the Study:
- To investigate the developmental expression patterns of calbindin-D28k and calretinin in the embryonic chick kidney.
- To determine the specific renal structures where these proteins are localized during ontogeny.
- To provide the first demonstration of calretinin presence in the developing kidney.
Main Methods:
- Immunohistochemical techniques were employed to detect calbindin-D28k and calretinin.
- Developing chick kidneys at various incubation stages were analyzed.
- Specific antibodies were used to visualize the distribution of the target proteins.
Main Results:
- Calbindin-D28k was detected from the 5th incubation day in mesonephric distal and connecting tubules, and Wolffian duct.
- Calretinin was identified from the 7th incubation day in similar structures, with its presence in the developing kidney being newly reported.
- Later developmental stages showed calretinin in some metanephric proximal tubules; glomeruli and Bowman's capsules remained negative.
- Differential immunoreactivity in the Wolffian duct correlated with its histological features.
Conclusions:
- The early expression of calbindin-D28k and calretinin in mesonephric distal tubules suggests their involvement in regulating calcium excretion.
- The distinct expression patterns indicate specific roles for these proteins in different segments of the developing kidney.
- This study establishes calretinin's presence in the developing kidney and highlights the proteins' potential roles in renal calcium homeostasis.
Abstract:
The ontogeny of two calcium-binding proteins (calbindin-D28k and calretinin) was studied by immunohistochemical techniques in developing chick kidney. This study showed the presence of calbindin on the 5th incubation day and calretinin on the 7th incubation day in mesonephric distal and connecting tubules, and in the medial wall of the Wolffian duct. At later stages, immunostaining for these two proteins, in particular for calretinin, was also demonstrated in some metanephric proximal tubules. Glomeruli and Bowman's capsules were negative both in the mesonephros and metanephros. The presence of calretinin in the developing kidney has thus been demonstrated for the first time. The early expression of calbindin and calretinin in mesonephric distal tubules suggests their role in regulating the final excretion of calcium. The different patterns of immunoreactivity of the walls of the Wolffian duct can be correlated with their different histogenetic and histological features.