Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Diode array detection in capillary electrophoresis

D N Heiger1, P Kaltenbach, H J Sievert

  • 1Hewlett-Packard Company, Wilmington, DE.

Electrophoresis
|October 1, 1994
PubMed
Summary

Diode array detection (DAD) in capillary electrophoresis (CE) enhances compound identification and peak purity. Optimized optical systems and bubble cells improve sensitivity and spectral data for quantitative analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Amino acid analysis by capillary electrophoresis electrospray ionization mass spectrometry.

Analytical chemistry·2000
Same author

Simultaneous determination of monosaccharides in glycoproteins by capillary electrophoresis.

Analytical biochemistry·1998
Same author

Separation of peptides and protein digests by capillary electrophoresis.

Methods in molecular biology (Clifton, N.J.)·1996
Same author

Sequence dependent migration behavior of double-stranded DNA in capillary electrophoresis.

Electrophoresis·1995
Same author

Identification of tryptophan and tyrosine residues in peptides separated by capillary electrophoresis by their second-derivative spectra using diode-array detection.

Journal of chromatography. A·1994
Same author

Separation of DNA fragments by capillary electrophoresis using replaceable linear polyacrylamide matrices.

Journal of chromatography. A·1993

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Diode array detection (DAD) offers advantages in capillary electrophoresis (CE) similar to high-performance liquid chromatography (HPLC).
  • Confirmation of compound identity and peak purity are critical in CE, requiring sensitive and specific detection methods.

Purpose of the Study:

  • To discuss the optimization of optical design and spectral fidelity for DAD in CE.
  • To present applications of CE and micellar electrokinetic capillary chromatography (MEKC) using DAD, focusing on quantitative analysis and purity verification.

Main Methods:

  • Utilizing an optimized optical system for DAD in CE.
  • Employing expanded pathlength capillaries with bubble cells to enhance sensitivity.
  • Applying spectral information for peak tracking and purity assessment in MEKC.

Main Results:

  • Optimized DAD provides sensitivity comparable to single or variable wavelength detectors.
  • Expanded pathlength capillaries (bubble cells) can improve sensitivity by three to five times.
  • Spectral data enables reliable compound identification, peak purity verification, and peak tracking in MEKC.

Conclusions:

  • DAD is a valuable tool for sensitive and specific detection in CE.
  • Optimized optical design and bubble cells significantly enhance CE performance.
  • Spectral information from DAD is crucial for robust quantitative analysis and quality control in CE and MEKC.

Related Experiment Videos