Mercuric chloride effects on rat renal redox enzymes activities: SOD protection

This study was done to determine the effect of mercuric chloride treatment on the redox cycle enzymes in rat kidney ex-vivo. Glutathione peroxidase (GSH-Px) and catalase (Cat) activities were measured in kidney homogenates from rats with different nonprotein sulfhydrils levels and different mercury content. The results indicated that GSH-Px activity was enhanced in mercury-treated rats in direct relationship with kidney mercury content, whereas Cat activity was increased in the presence of the highest mercury kidney content obtained. Superoxide dismutase (SOD) was administered to rats prior to mercury chloride injection and renal function, development of lipid peroxidation and renal glutathione level were measured 1 h later. Renal function, renal glutathione, and renal lipid peroxidation production were maintained similar to control values. Moreover, SOD pretreatment also protected kidney from mercuric chloride histological alterations observed 24 h post mercury treatment. Thus, an inhibition of renal redox cycle enzymes "in vivo," did not appear to be an important determinant of the increased lipid peroxidation observed during mercuric chloride nephrotoxicity.