Primary cultures of neurons for testing neuroprotective drug effects

Primary cultures of neurons are widely used for the investigation of pathomechanisms of neuronal damage and for the evaluation of neuroprotective drug effects. The present paper gives a short survey of frequently used primary neuronal culture systems and of experimental measures for the induction of defined neuronal damage with particular respect to the pathomechanisms of cerebral ischemia. Neuroprotective drug effects as achieved under these conditions are reviewed, and the neuroprotective effects of glutamate antagonists, radical scavengers, and neural growth factors are discussed in some more detail.