Related Experiment Videos
cDNA approaches to isolation of the mouse mutant weaver gene
M E Hodes1, S R Dlouhy, J J Wei
1Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis 46202.
Neurochemical Research
|November 1, 1994
Abstract:
The mouse autosomal recessive mutant gene weaver (wv) results in abnormalities in cerebellum, substantia nigra and testis. Although a substracted cDNA library prepared by removing P31 (wv/wv) sequences from a P1 (wv/+) library should contain mainly nonrepetitive neonatal sequences, unfortunately, repetitive sequences still appear during screening. Two clones, one repetitive, the other not, are used to illustrate the problems encountered in attempting to isolate the weaver gene from a substrated cDNA library.