Related Experiment Video

Updated: Aug 4, 2026

Ferric Chloride-induced Thrombosis Mouse Model on Carotid Artery and Mesentery Vessel
07:26

Ferric Chloride-induced Thrombosis Mouse Model on Carotid Artery and Mesentery Vessel

Published on: June 29, 2015

Effect of platelet activating factor antagonists in different models of thrombosis

P Seth1, R Kumari, M Dikshit

  • 1Pharmacology Division, Central Drug Research Institute, Lucknow, India.

Thrombosis Research
|December 15, 1994
PubMed

Insights

Three platelet-activating factor (PAF) antagonists showed dose-dependent protection against thrombosis in mice and cats. However, these PAF antagonists were ineffective in preventing thrombosis in rats, indicating species-specific effects.

Area of Science:

  • Pharmacology
  • Thrombosis Research
  • Cardiovascular Science

Background:

  • Platelet-activating factor (PAF) is a potent lipid mediator involved in inflammation and thrombosis.
  • PAF antagonists are investigated for their potential to prevent thrombotic events.

Purpose of the Study:

  • To evaluate the efficacy of three PAF antagonists (SR-27417, BN-50739, and BN-52021) in preclinical models of thrombosis.
  • To assess potential species-specific differences in the effectiveness of these antagonists.

Main Methods:

  • Thrombosis was induced in mice via collagen and adrenaline infusion.
  • Rat models involved thrombosis induction by metallic wire insertion into the inferior vena cava.
  • Cat models assessed thrombus formation in an extracorporeal shunt.

Main Results:

  • PAF antagonists demonstrated dose-dependent protection against pulmonary thromboembolism in mice.
  • Effective protection against thrombosis was observed in cats using the extracorporeal shunt model.
  • No significant antithrombotic effect was observed in rats, even at the highest doses tested.

Conclusions:

  • PAF antagonists exhibit varying efficacy across different species.
  • SR-27417, BN-50739, and BN-52021 show promise in managing thrombosis in mice and cats.
  • Further research is needed to understand the lack of efficacy in rats and potential clinical applications.

More Related Videos

Ferric Chloride-induced Murine Thrombosis Models10:37

Ferric Chloride-induced Murine Thrombosis Models

Published on: September 5, 2016

Microfluidics in Assessing Platelet Function06:47

Microfluidics in Assessing Platelet Function

Published on: November 8, 2024

Related Concept Videos

Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Formation of the Platelet Plug01:22

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
Clot Retraction and Fibrinolysis01:16

Clot Retraction and Fibrinolysis

After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.
Venous Thrombosis III: Interprofessional Care01:29

Venous Thrombosis III: Interprofessional Care

Venous thrombosis requires effective prevention and treatment strategies to improve patient outcomes and reduce potential complications.Prevention StrategiesHealthcare providers must prioritize preventing venous thromboembolism (VTE) for all adult patients upon admission. Interventions depend on bleeding and thrombosis risk, medical history, current medications, diagnoses, planned procedures, and patient preferences. Patients on bed rest should change positions every two hours and, if not...